Thrombospondin type-1 domain-containing 7A in idiopathic membranous nephropathy.

ABSTRACT:

Background

Idiopathic membranous nephropathy is an autoimmune disease. In approximately 70% of patients, it is associated with autoantibodies against the phospholipase A2 receptor 1 (PLA2R1). Antigenic targets in the remaining patients are unknown.

Methods

Using Western blotting, we screened serum samples from patients with idiopathic membranous nephropathy, patients with other glomerular diseases, and healthy controls for antibodies against human native glomerular proteins. We partially purified a putative new antigen, identified this protein by means of mass spectrometry of digested peptides, and validated the results by analysis of recombinant protein expression, immunoprecipitation, and immunohistochemical analysis.

Results

Serum samples from 6 of 44 patients in a

SUBMITTER: Tomas NM 

PROVIDER: S-EPMC4278759 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature