Quantitative detection of circulating nucleophosmin mutations DNA in the plasma of patients with acute myeloid leukemia.

ABSTRACT:

Objective

The aim of this study was to quantify the copies of circulating nucleophosmin (NPM) mutations DNA in the plasma of patients with acute myeloid leukemia (AML) and to explore the association of circulating NPM mutation levels with clinical characteristics.

Design and methods

The presence of NPM mutations in 100 Chinese patients newly diagnosed with AML were identified by RT-PCR and sequencing analysis. Copies of circulating NPM mutation A (NPM mut.A) DNA in the plasma of mutation-positive cases were quantified by real-time quantitative PCR (qRT-PCR). Furthermore, the association of circulating NPM mutation levels and clinical characteristics was analyzed.

Results

NPM mutations were identified in 37 of the 100 patients and all cases were NPM mut.A. The circulat

SUBMITTER: Quan J 

PROVIDER: S-EPMC4278871 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature