Comparison of custom capture for targeted next-generation DNA sequencing.

ABSTRACT: Targeted, capture-based DNA sequencing is a cost-effective method to focus sequencing on a coding region or other customized region of the genome. There are multiple targeted sequencing methods available, but none has been systematically investigated and compared. We evaluated four commercially available custom-targeted DNA technologies for next-generation sequencing with respect to on-target sequencing, uniformity, and ability to detect single-nucleotide variations (SNVs) and copy number variations. The technologies that used sonication for DNA fragmentation displayed impressive uniformity of capture, whereas the others had shorter preparation times, but sacrificed uniformity. One of those technologies, which uses transposase for DNA fragmentation, has a drawback requiring sample pooling,

SUBMITTER: Samorodnitsky E 

PROVIDER: S-EPMC4279426 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature