Fabrication and evaluation of a micro(bio)sensor array chip for multiple parallel measurements of important cell biomarkers.

ABSTRACT: This report describes the design and development of an integrated electrochemical cell culture monitoring system, based on enzyme-biosensors and chemical sensors, for monitoring indicators of mammalian cell metabolic status. MEMS technology was used to fabricate a microwell-format silicon platform including a thermometer, onto which chemical sensors (pH, O2) and screen-printed biosensors (glucose, lactate), were grafted/deposited. Microwells were formed over the fabricated sensors to give 5-well sensor strips which were interfaced with a multipotentiostat via a bespoke connector box interface. The operation of each sensor/biosensor type was examined individually, and examples of operating devices in five microwells in parallel, in either potentiometric (pH sensing) or amperometric (glucose

SUBMITTER: Pemberton RM 

PROVIDER: S-EPMC4279497 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature