Modulation of lipopolysaccharide-induced neuronal response by activation of the enteric nervous system.

ABSTRACT:

Background

Evidence continues to mount concerning the importance of the enteric nervous system (ENS) in controlling numerous intestinal functions in addition to motility and epithelial functions. Nevertheless, little is known concerning the direct participation of the ENS in the inflammatory response of the gut during infectious or inflammatory insults. In the present study we analyzed the ENS response to bacterial lipopolysaccharide, in particular the production of a major proinflammatory cytokine, tumor necrosis factor-alpha (TNF-α).

Methods

TNF-α expression (measured by qPCR, quantitative Polymerase Chain Reaction) and production (measured by ELISA) were measured in human longitudinal muscle-myenteric plexus (LMMP) and rat ENS primary cultures (rENSpc). They were either t

SUBMITTER: Coquenlorge S 

PROVIDER: S-EPMC4279994 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature