Expression of the dermatomyositis autoantigen transcription intermediary factor 1γ in regenerating muscle.

ABSTRACT:

Objective

Autoantibodies against transcription intermediary factor 1γ (TIF1γ) are found in many patients with dermatomyositis (DM). Although TIF1γ is known to play a role in the differentiation of other tissues, its functional role in muscle regeneration has not been elucidated. This study was undertaken to explore the regulation and functional role of this protein during muscle differentiation and regeneration.

Methods

TIF1γ expression was analyzed in human muscle biopsy specimens using immunofluorescence microscopy. Immunofluorescence microscopy and immunoblotting analyses were used to study TIF1γ expression in a mouse model of muscle injury and repair. The effect of premature TIF1γ silencing on muscle differentiation was studied in cultured mouse myoblasts.

Results

SUBMITTER: Mohassel P 

PROVIDER: S-EPMC4280343 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature