Signals from the surface modulate differentiation of human pluripotent stem cells through glycosaminoglycans and integrins.

ABSTRACT: The fate decisions of human pluripotent stem (hPS) cells are governed by soluble and insoluble signals from the microenvironment. Many hPS cell differentiation protocols use Matrigel, a complex and undefined substrate that engages multiple adhesion and signaling receptors. Using defined surfaces programmed to engage specific cell-surface ligands (i.e., glycosaminoglycans and integrins), the contribution of specific matrix signals can be dissected. For ectoderm and motor neuron differentiation, peptide-modified surfaces that can engage both glycosaminoglycans and integrins are effective. In contrast, surfaces that interact selectively with glycosaminoglycans are superior to Matrigel in promoting hPS cell differentiation to definitive endoderm and mesoderm. The modular surfaces were used to

SUBMITTER: Wrighton PJ 

PROVIDER: S-EPMC4280649 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature