Fine spatiotemporal activity in contracting myometrium revealed by motion-corrected calcium imaging.

ABSTRACT: Successful childbirth depends on the occurrence of precisely coordinated uterine contractions during labour. Calcium indicator fluorescence imaging is one of the main techniques for investigating the mechanisms governing this physiological process and its pathologies. The effective spatiotemporal resolution of calcium signals is, however, limited by the motion of contracting tissue: structures of interest in the order of microns can move over a hundred times their width during a contraction. The simultaneous changes in local intensity and tissue configuration make motion tracking a non-trivial problem in image analysis and confound many of the standard techniques. This paper presents a method that tracks local motion throughout the tissue and allows for the almost complete removal of motio

SUBMITTER: Loftus FC 

PROVIDER: S-EPMC4280886 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature