Cell-specific STORM super-resolution imaging reveals nanoscale organization of cannabinoid signaling.

ABSTRACT: A major challenge in neuroscience is to determine the nanoscale position and quantity of signaling molecules in a cell type- and subcellular compartment-specific manner. We developed a new approach to this problem by combining cell-specific physiological and anatomical characterization with super-resolution imaging and studied the molecular and structural parameters shaping the physiological properties of synaptic endocannabinoid signaling in the mouse hippocampus. We found that axon terminals of perisomatically projecting GABAergic interneurons possessed increased CB1 receptor number, active-zone complexity and receptor/effector ratio compared with dendritically projecting interneurons, consistent with higher efficiency of cannabinoid signaling at somatic versus dendritic synapses. Furthe

SUBMITTER: Dudok B 

PROVIDER: S-EPMC4281300 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature