Influence of the lipid membrane environment on structure and activity of the outer membrane protein Ail from Yersinia pestis.

ABSTRACT: The surrounding environment has significant consequences for the structural and functional properties of membrane proteins. While native structure and function can be reconstituted in lipid bilayer membranes, the detergents used for protein solubilization are not always compatible with biological activity and, hence, not always appropriate for direct detection of ligand binding by NMR spectroscopy. Here we describe how the sample environment affects the activity of the outer membrane protein Ail (attachment invasion locus) from Yersinia pestis. Although Ail adopts the correct β-barrel fold in micelles, the high detergent concentrations required for NMR structural studies are not compatible with the ligand binding functionality of the protein. We also describe preparations of Ail embedded i

SUBMITTER: Ding Y 

PROVIDER: S-EPMC4281492 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature