Effect of phosphatidylcholine on the level expression of plc genes of Aspergillus fumigatus by real time PCR method and investigation of these genes using bioinformatics analysis.

ABSTRACT: BACKGROUND AND OBJECTIVES:Phosphlipases are a group of enzymes that breakdown phosphatidylcholine (phospholipids) molecules producing second products. These produced products have a divers role in the cell like signal transduction and digestion in humans. In this research the effect of phosphatidylcholine on the expression of plc genes of A. fumigatus was studied. The plc genes of this fungus were also interrogated using bioinformatics studies. MATERIALS AND METHODS:Real-time PCR was performed to study the expression of plc genes and these genes were interrogated using bioinformatics studies. RESULTS:There was more significant expression for all three plc genes when A. fumigatus was grown on the presence of phosphatidylcholine in the medium. The sequence of plc genes of A. fumigatus was al

SUBMITTER: Dehghan-Noodeh A 

PROVIDER: S-EPMC4281657 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature