Proteomics analyses of Bacillus subtilis after treatment with plumbagin, a plant-derived naphthoquinone.

ABSTRACT: Infectious diseases and increasing antibiotic resistance among diverse classes of microbes are global health concerns and a prime focus of omics systems science applications in novel drug discovery. Plumbagin is a plant-derived naphthoquinone, a natural product that exhibits antibacterial activity against gram-positive bacteria. In the present study, we investigated the antimicrobial effects of plumbagin against Bacillus subtilis using two complementary proteomics techniques: two-dimensional electrophoresis (2-DE) and isobaric tag for relative and absolute quantification (iTRAQ). Comparative quantitative proteomics analysis of plumbagin treated and untreated control samples identified differential expression of 230 proteins (1% FDR, 1.5 fold-change and ≥2 peptides) in B. subtilis after plu

SUBMITTER: Reddy PJ 

PROVIDER: S-EPMC4281856 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature