CRISPR-Cas9-mediated single-gene and gene family disruption in Trypanosoma cruzi.

ABSTRACT:

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Trypanosoma cruzi is a protozoan parasite of humans and animals, affecting 10 to 20 million people and innumerable animals, primarily in the Americas. Despite being the largest cause of infection-induced heart disease worldwide, even among the neglected tropical diseases (NTDs) T. cruzi is considered one of the least well understood and understudied. The genetic complexity of T. cruzi as well as the limited set of efficient techniques for genome engineering contribute significantly to the relative lack of progress in and understanding of this pathogen. Here, we adapted the CRISPR-Cas9 system for the genetic engineering of T. cruzi, demonstrating rapid and efficient knockout of multiple endogenous genes, including essential genes. We observed that in the absence of a temp

SUBMITTER: Peng D 

PROVIDER: S-EPMC4281920 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature