A functional glycogen biosynthesis pathway in Lactobacillus acidophilus: expression and analysis of the glg operon.

ABSTRACT: Glycogen metabolism contributes to energy storage and various physiological functions in some prokaryotes, including colonization persistence. A role for glycogen metabolism is proposed on the survival and fitness of Lactobacillus acidophilus, a probiotic microbe, in the human gastrointestinal environment. L. acidophilus NCFM possesses a glycogen metabolism (glg) operon consisting of glgBCDAP-amy-pgm genes. Expression of the glg operon and glycogen accumulation were carbon source- and growth phase-dependent, and were repressed by glucose. The highest intracellular glycogen content was observed in early log-phase cells grown on trehalose, which was followed by a drastic decrease of glycogen content prior to entering stationary phase. In raffinose-grown cells, however, glycogen accumulation

SUBMITTER: Goh YJ 

PROVIDER: S-EPMC4282360 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature