A sporulation-specific, sigF-dependent protein, SspA, affects septum positioning in Streptomyces coelicolor.

ABSTRACT: The RNA polymerase sigma factor SigF controls late development during sporulation in the filamentous bacterium Streptomyces coelicolor. The only known SigF-dependent gene identified so far, SCO5321, is found in the biosynthetic cluster encoding spore pigment synthesis. Here we identify the first direct target for SigF, the gene sspA, encoding a sporulation-specific protein. Bioinformatic analysis suggests that SspA is a secreted lipoprotein with two PepSY signature domains. The sspA deletion mutant exhibits irregular sporulation septation and altered spore shape, suggesting that SspA plays a role in septum formation and spore maturation. The fluorescent translational fusion protein SspA-mCherry localized first to septum sites, then subsequently around the surface of the spores. Both SspA p

SUBMITTER: Tzanis A 

PROVIDER: S-EPMC4282423 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature