Function of mutant and wild-type plexinb1 in prostate cancer cells.

ABSTRACT:

Background

Semaphorins act as chemotactic cues for cell movement via their transmembrane receptors, plexins. Somatic missense mutations in the plexinB1 gene coupled with overexpression of the protein frequently occur in prostate tumors, indicating a role for plexinB1 in the pathogenesis of prostate cancer. However, the effect of semaphorin/plexin signaling is highly context dependent and whether plexinB1 acts as an inducer or inhibitor of prostate tumor progression in this context is not known.

Methods

The response of prostate cancer cell lines to plexinB1 activation was assessed in migration, invasion, proliferation and protein phosphorylation assays. Expression was assessed by quantitative RTPCR and immunoblotting.

Results

Different prostate cancer cell lines respon

SUBMITTER: Damola A 

PROVIDER: S-EPMC4282548 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature