Induction of autophagy supports the bioenergetic demands of quiescent muscle stem cell activation.

ABSTRACT: The exit of a stem cell out of quiescence into an activated state is characterized by major metabolic changes associated with increased biosynthesis of proteins and macromolecules. The regulation of this transition is poorly understood. Using muscle stem cells, or satellite cells (SCs), we found that autophagy, which catabolizes intracellular contents to maintain proteostasis and to produce energy during nutrient deprivation, was induced during SC activation. Inhibition of autophagy suppressed the increase in ATP levels and delayed SC activation, both of which could be partially rescued by exogenous pyruvate as an energy source, suggesting that autophagy may provide nutrients necessary to meet bioenergetic demands during this critical transition from quiescence to activation. We found that

SUBMITTER: Tang AH 

PROVIDER: S-EPMC4282556 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature