Characterising the expression and function of CCL28 and its corresponding receptor, CCR10, in RA pathogenesis.

ABSTRACT:

Objective

This study was conducted to determine the expression pattern, regulation and function of CCL28 and CCR10 in rheumatoid arthritis (RA) pathogenesis.

Methods

Expression of CCL28 and CCR10 was assessed in RA compared with other arthritis synovial tissues (STs) or fluids (SFs) by histology or ELISA. The factors modulating CCL28 and CCR10 expression were identified in RA myeloid and endothelial cells by ELISA, FACS and Western blotting. The mechanism by which CCL28 ligation promotes RA angiogenesis was examined in control and CCR10-knockdown endothelial cell chemotaxis and capillary formation.

Results

CCL28 and/or CCR10 expression levels were accentuated in STs and SFs of patients with joint disease compared with normal controls and they were predominately coexpr

SUBMITTER: Chen Z 

PROVIDER: S-EPMC4282625 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature