Identification of a novel gene fusion (BMX-ARHGAP) in gastric cardia adenocarcinoma.

ABSTRACT:

Background

Gastric cardia adenocarcinoma (GCA) is one of the major causes of cancer related mortality worldwide. We aim to provide new understanding in the pathogenesis of GCA through investigations on gene expression alterations.

Methods

We preformed RNA-Seq for one pair of GCA and matched non-tumor tissues. Differentially expressed genes (DEGs) and fusion genes were acquired. PCR and gel analysis in additional 14 pairs of samples were performed to validate the chimeric transcripts.

Results

1590 up-regulated and 709 down-regulated genes were detected. Functional analysis revealed that these DEGs were significantly overrepresented in gene ontology items of cell cycle, tumor invasion and proliferation. Moreover, we firstly discovered 3 fusion genes in GCA, including BM

SUBMITTER: Xu X 

PROVIDER: S-EPMC4282731 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature