Systematic, spatial imaging of large multimolecular assemblies and the emerging principles of supramolecular order in biological systems.

ABSTRACT: Understanding biological systems at the level of their relational (emergent) molecular properties in functional protein networks relies on imaging methods, able to spatially resolve a tissue or a cell as a giant, non-random, topologically defined collection of interacting supermolecules executing myriads of subcellular mechanisms. Here, the development and findings of parameter-unlimited functional super-resolution microscopy are described-a technology based on the fluorescence imaging cycler (IC) principle capable of co-mapping thousands of distinct biomolecular assemblies at high spatial resolution and differentiation (<40 nm distances). It is shown that the subcellular and transcellular features of such supermolecules can be described at the compositional and constitutional levels; that

SUBMITTER: Schubert W 

PROVIDER: S-EPMC4283051 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature