Blocking the expression of syntaxin 4 interferes with initial phagocytosis of Brucella melitensis in macrophages.

ABSTRACT: Brucella melitensis is the Brucella species most frequently associated with brucellosis in humans. It is also the causative agent of the disease in goats and other ruminants. Although significant aspects of the pathogenesis of infection by this intracellular pathogen have been clarified, several events during invasion of host cells remain to be elucidated. In this study, infections of human macrophages from the THP-1 monocyte cell line were conducted with B. melitensis Bm133 wild-type strain and a strain of Salmonella serovar Enteritidis as a control. A multiplicity of infection of 100 was used in trials focused on defining the relative expression of syntaxin 4 (STX4), a soluble N-ethylmaleimide-sensitive factor attachment protein receptor, in the early events of phagocytosis (at 15, 30, 4

SUBMITTER: Castaneda-Ramirez A 

PROVIDER: S-EPMC4283232 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature