Biophysical characterization of Chlamydia trachomatis CT584 supports its potential role as a type III secretion needle tip protein.

ABSTRACT: Chlamydia are obligate intracellular bacterial pathogens that cause a variety of diseases. Like many Gram-negative bacteria, they employ type III secretion systems (T3SS) for invasion, establishing and maintaining their unique intracellular niche, and possibly cellular exit. Computational structure prediction indicated that ORF CT584 is homologous to other T3SS needle tip proteins. Tip proteins have been shown to be localized to the extracellular end of the T3SS needle and play a key role in controlling secretion of effector proteins. We have previously demonstrated that T3SS needle tip proteins from different bacteria share many biophysical characteristics. To support the hypothesis that CT584 is a T3SS needle tip protein, biophysical properties of CT584 were explored as a function of pH

SUBMITTER: Markham AP 

PROVIDER: S-EPMC4285778 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature