From metabolomics to fluxomics: a computational procedure to translate metabolite profiles into metabolic fluxes.

ABSTRACT: We describe a believed-novel procedure for translating metabolite profiles (metabolome) into the set of metabolic fluxes (fluxome) from which they originated. Methodologically, computational modeling is integrated with an analytical platform comprising linear optimization, continuation and dynamic analyses, and metabolic control. The procedure was tested with metabolite profiles obtained from ex vivo mice Langendorff-heart preparations perfused with glucose. The metabolic profiles were analyzed using a detailed kinetic model of the glucose catabolic pathways including glycolysis, pentose phosphate (PP), glycogenolysis, and polyols to translate the glucose metabolome of the heart into the fluxome. After optimization, the ability of the model to simulate the initial metabolite profile was co

SUBMITTER: Cortassa S 

PROVIDER: S-EPMC4286601 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature