ClaR--a novel key regulator of cellobiose and lactose metabolism in Lactococcus lactis IL1403.

ABSTRACT: In a number of previous studies, our group has discovered an alternative pathway for lactose utilization in Lactococcus lactis that, in addition to a sugar-hydrolyzing enzyme with both P-β-glucosidase and P-β-galactosidase activity (BglS), engages chromosomally encoded components of cellobiose-specific PTS (PTS(Cel-Lac)), including PtcA, PtcB, and CelB. In this report, we show that this system undergoes regulation via ClaR, a novel activator protein from the RpiR family of transcriptional regulators. Although RpiR proteins are widely distributed among lactic acid bacteria, their roles have yet to be confirmed by functional assays. Here, we show that ClaR activity depends on intracellular cellobiose-6-phosphate availability, while other sugars such as glucose or galactose have no influence

SUBMITTER: Aleksandrzak-Piekarczyk T 

PROVIDER: S-EPMC4286628 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature