Genomic architecture of histone 3 lysine 27 trimethylation during late ovine skeletal muscle development.

ABSTRACT: The ruminant developmental transition from late foetus to lamb is associated with marked changes in skeletal muscle structure and function that reflect programming for new physiological demands following birth. To determine whether epigenetic changes are involved in this transition, we investigated the genomic architecture of the chromatin modification, histone 3 lysine 27 trimethylation (H3K27me3), which typically regulates early life developmental processes; however, its role in later life processes is unclear. Chromatin immunoprecipitation coupled with next-generation sequencing was used to map H3K27me3 nucleosomes in ovine longissimus lumborum skeletal muscle at 100 days of gestation and 12 weeks post-partum. In both states, H3K27me3 modification was associated with genes, transcriptio

SUBMITTER: Byrne K 

PROVIDER: S-EPMC4286725 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature