Small non-coding RNA profiling and the role of piRNA pathway genes in the protection of chicken primordial germ cells.

ABSTRACT:

Background

Genes, RNAs, and proteins play important roles during germline development. However, the functions of non-coding RNAs (ncRNAs) on germline development remain unclear in avian species. Recent high-throughput techniques have identified several classes of ncRNAs, including micro RNAs (miRNAs), small-interfering RNAs (siRNAs), and PIWI-interacting RNAs (piRNAs). These ncRNAs are functionally important in the genome, however, the identification and annotation of ncRNAs in a genome is challenging. The aim of this study was to identify different types of small ncRNAs particularly piRNAs, and the role of piRNA pathway genes in the protection of chicken primordial germ cells (PGCs).

Results

At first, we performed next-generation sequencing to identify ncRNAs in chicken PGC

SUBMITTER: Rengaraj D 

PROVIDER: S-EPMC4286946 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature