Peptidases compartmentalized to the Ascaris suum intestinal lumen and apical intestinal membrane.

ABSTRACT: The nematode intestine is a tissue of interest for developing new methods of therapy and control of parasitic nematodes. However, biological details of intestinal cell functions remain obscure, as do the proteins and molecular functions located on the apical intestinal membrane (AIM), and within the intestinal lumen (IL) of nematodes. Accordingly, methods were developed to gain a comprehensive identification of peptidases that function in the intestinal tract of adult female Ascaris suum. Peptidase activity was detected in multiple fractions of the A. suum intestine under pH conditions ranging from 5.0 to 8.0. Peptidase class inhibitors were used to characterize these activities. The fractions included whole lysates, membrane enriched fractions, and physiological- and 4 molar urea-perfusat

SUBMITTER: Jasmer DP 

PROVIDER: S-EPMC4287503 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature