ASAR15, A cis-acting locus that controls chromosome-wide replication timing and stability of human chromosome 15.

ABSTRACT: DNA replication initiates at multiple sites along each mammalian chromosome at different times during each S phase, following a temporal replication program. We have used a Cre/loxP-based strategy to identify cis-acting elements that control this replication-timing program on individual human chromosomes. In this report, we show that rearrangements at a complex locus at chromosome 15q24.3 result in delayed replication and structural instability of human chromosome 15. Characterization of this locus identified long, RNA transcripts that are retained in the nucleus and form a "cloud" on one homolog of chromosome 15. We also found that this locus displays asynchronous replication that is coordinated with other random monoallelic genes on chromosome 15. We have named this locus ASynchronous re

SUBMITTER: Donley N 

PROVIDER: S-EPMC4287527 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature