Beta cell regeneration after single-round immunological destruction in a mouse model.

ABSTRACT:

Aims/hypothesis

Achieving a better understanding of beta cell regeneration after immunological destruction is crucial for the development of immunotherapy approaches for type 1 diabetes. In previous type 1 diabetes models, sustained immune activation eliminates regenerating beta cells, thus limiting the study of the regenerative capacity of beta cells upon immunological destruction. Here, we employed an adeno-associated virus 8 (AAV8) vector for beta cell-targeted overexpression of a foreign antigen to induce single-round immunological destruction of existing beta cells.

Methods

Young and aged C57BL/6J mice were treated with AAV8 vectors expressing the foreign antigen luciferase. Islet inflammation and regeneration was observed at 3, 6, 10 and 22 weeks post-AAV delivery.

SUBMITTER: Tonne JM 

PROVIDER: S-EPMC4287683 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature