Quantitative proteomics reveals a role for epigenetic reprogramming during human monocyte differentiation.

ABSTRACT: The differentiation of monocytes into macrophages and dendritic cells involves mechanisms for activation of the innate immune system in response to inflammatory stimuli, such as pathogen infection and environmental cues. Epigenetic reprogramming is thought to play an important role during monocyte differentiation. Complementary to cell surface markers, the characterization of monocytic cell lineages by mass spectrometry based protein/histone expression profiling opens a new avenue for studying immune cell differentiation. Here, we report the application of mass spectrometry and bioinformatics to identify changes in human monocytes during their differentiation into macrophages and dendritic cells. Our data show that linker histone H1 proteins are significantly down-regulated during monocyte

SUBMITTER: Nicholas D 

PROVIDER: S-EPMC4288251 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature