CysTMTRAQ-An integrative method for unbiased thiol-based redox proteomics.

ABSTRACT: Protein redox regulation plays important roles in many biological processes. Protein cysteine thiols are sensitive to redox changes and may function as redox switches, which turn signaling and metabolic pathways on or off to ensure speedy responses to environmental stimuli or stresses. Here we report a novel integrative proteomics method called cysTMTRAQ that combines two types of isobaric tags, cysteine tandem mass tags and isobaric tag for relative and absolute quantification, in one experiment. The method not only enables simultaneous analysis of cysteine redox changes and total protein level changes, but also allows the determination of bona fide redox modified cysteines in proteins through the correction of protein turnover. Overlooking the factor of protein-level changes in the cours

SUBMITTER: Parker J 

PROVIDER: S-EPMC4288258 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature