Proteomes of host cell membranes modified by intracellular activities of Salmonella enterica.

ABSTRACT: Intracellular pathogens need to establish a growth-stimulating host niche for survival and replication. A unique feature of the gastrointestinal pathogen Salmonella enterica serovar Typhimurium is the creation of extensive membrane networks within its host. An understanding of the origin and function of these membranes is crucial for the development of new treatment strategies. However, the characterization of this compartment is very challenging, and only fragmentary knowledge of its composition and biogenesis exists. Here, we describe a new proteome-based approach to enrich and characterize Salmonella-modified membranes. Using a Salmonella mutant strain that does not form this unique membrane network as a reference, we identified a high-confidence set of host proteins associated with Sal

SUBMITTER: Vorwerk S 

PROVIDER: S-EPMC4288265 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature