Glioma-derived versican promotes tumor expansion via glioma-associated microglial/macrophages Toll-like receptor 2 signaling.

ABSTRACT:

Background

Accumulation and infiltration of microglia/brain macrophages around and into glioma tissue promote tumor invasion and expansion. One tumor-promoting mechanism of microglia/brain macrophages is upregulation of membrane type 1 matrix metalloprotease (MT1-MMP), which promotes the degradation of extracellular matrix. MT1-MMP upregulation is induced by soluble factors released by glioma cells activating microglial Toll-like receptor 2 (TLR2).

Methods

Versican identified by proteomics was silenced in glioma cells by short interference RNA and short hairpin RNA approaches and studied in vitro and after injection into mouse brains or organotypic brain slices.

Results

The splice variants V0/V1 of the endogenous TLR2 ligand versican are highly expressed in mouse and

SUBMITTER: Hu F 

PROVIDER: S-EPMC4288527 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature