FlgM is secreted by the flagellar export apparatus in Bacillus subtilis.

ABSTRACT: The bacterial flagellum is assembled from over 20 structural components, and flagellar gene regulation is morphogenetically coupled to the assembly state by control of the anti-sigma factor FlgM. In the Gram-negative bacterium Salmonella enterica, FlgM inhibits late-class flagellar gene expression until the hook-basal body structural intermediate is completed and FlgM is inhibited by secretion from the cytoplasm. Here we demonstrate that FlgM is also secreted in the Gram-positive bacterium Bacillus subtilis and is degraded extracellularly by the proteases Epr and WprA. We further demonstrate that, like in S. enterica, the structural genes required for the flagellar hook-basal body are required for robust activation of σ(D)-dependent gene expression and efficient secretion of FlgM. Finally,

SUBMITTER: Calvo RA 

PROVIDER: S-EPMC4288692 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature