Wnt signaling is involved in 6-benzylthioinosine-induced AML cell differentiation.

ABSTRACT:

Background

We previously demonstrated that 6-benzylthioinosine (6-BT) could induce the differentiation of a subset of acute myeloid leukemia (AML) cell lines and primary AML cells regardless of their cytogenetics. In this study we investigated whether Wnt signaling pathways played roles in 6-BT-induced differentiation of AML cells.

Methods

We induced differentiation of HL-60 leukemic cells and primary AML cells in vitro using 6-BT. Real-time PCR (qPCR), western blot, and luciferase assays were used to examine the molecules' expression and biological activity in canonical and noncanonical Wnt signaling pathways. AML cell differentiation was measured by the Nitroblue tetrozolium (NBT) reduction assay.

Results

6-BT regulated the expression of both canonical and non-canon

SUBMITTER: Zang S 

PROVIDER: S-EPMC4289047 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature