Map of open and closed chromatin domains in Drosophila genome.

ABSTRACT:

Background

Chromatin compactness has been considered a major determinant of gene activity and has been associated with specific chromatin modifications in studies on a few individual genetic loci. At the same time, genome-wide patterns of open and closed chromatin have been understudied, and are at present largely predicted from chromatin modification and gene expression data. However the universal applicability of such predictions is not self-evident, and requires experimental verification.

Results

We developed and implemented a high-throughput analysis for general chromatin sensitivity to DNase I which provides a comprehensive epigenomic assessment in a single assay. Contiguous domains of open and closed chromatin were identified by computational analysis of the data, and

SUBMITTER: Milon B 

PROVIDER: S-EPMC4289254 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature