Anti-cancer effect and gene modulation of ET-743 in human biliary tract carcinoma preclinical models.

ABSTRACT:

Background

Standard chemotherapy in unresectable biliary tract carcinoma (BTC) patients is based on gemcitabine combined with platinum derivatives. However, primary or acquired resistance is inevitable and no second-line chemotherapy is demonstrated to be effective. Thus, there is an urgent need to identify new alternative (chemo)therapy approaches.

Methods

We evaluated the mechanism of action of ET-743 in preclinical models of BTC. Six BTC cell lines (TFK-1, EGI-1, TGBC1, WITT, KMCH, HuH28), two primary cell cultures derived from BTC patients, the EGI-1 and a new established BTC patient-derived xenografts, were used as preclinical models to investigate the anti-tumor activity of ET-743 in vitro and in vivo. Gene expression profiling was also analyzed upon ET-743 treatment i

SUBMITTER: Peraldo-Neia C 

PROVIDER: S-EPMC4289395 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature