Short hairpin ribonucleic acid constructs targeting insulin-like growth factor binding protein-3 rehabilitated decreased testosterone concentrations in diabetic rats.

ABSTRACT:

Background

The aim of this study was to determine if shRNA constructs targeting insulin-like growth factor binding protein-3 can rehabilitate decreased serum testosterone concentrations in streptozotocin-induced diabetic rats.

Material/methods

After 12 weeks of intracavernous administration of IGFBP-3 shRNA, intracavernous pressure responses to electrical stimulation of cavernous nerves were evaluated. The expression of IGFBP-3 at mRNA and protein levels was detected by quantitative real-time PCR analysis and Western blot, respectively. The concentrations of serum testosterone and cavernous cyclic guanosine monophosphate were detected by enzyme-linked immunosorbent assay.

Results

After 12 weeks of intracavernous administration of IGFBP-3 shRNA, the cavernosal pressure

SUBMITTER: Zhou ZY 

PROVIDER: S-EPMC4289482 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature