Efficient siRNA delivery using a polyamidoamine dendrimer with a modified pentaerythritol core.

ABSTRACT:

Purpose

Delivery of siRNA into cells remains a critical challenge. Our lab has shown a novel polyamidoamine (PAMAM) dendrimer with modified pentaerythritol derivative core (PD dendrimer) to exhibit high plasmid DNA transfection efficiency and low cytotoxicity. Here, we evaluate PD dendrimer as a siRNA carrier.

Methods

Agarose gel electrophoresis and AFM were used to confirm formation of generation 5 (G5)-PD dendrimer/siRNA nanoparticles (NPs). G5 PD dendrimer/anti-luciferase siRNA NPs were used to transfect SK Hep-1 cells with stable luciferase expression. Effects of various endocytic pathway inhibitors on uptake of G5 PD dendrimer/siRNA NPs in SK Hep-1 cells were also investigated.

Results

Agarose gel electrophoresis indicated that G5 PD dendrimer and siRNA formed NP

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC4289905 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature