PPemd26, an anthraquinone derivative, suppresses angiogenesis via inhibiting VEGFR2 signalling.

ABSTRACT:

Background and purpose

Angiogenesis contributes to coronary heart disease, immune disorders and numerous malignancies. VEGF-A and its receptors (VEGFRs) play a pivotal role in regulating angiogenesis. In an effort to discover more effective inhibitors of tumour angiogenesis, we have analysed the actions of a novel anthraquinone derivative, PPemd26, and explored its anti-angiogenic mechanisms.

Experimental approach

The effects of PPemd26 were evaluated in vitro using HUVEC cultures to assess proliferation, migration, invasion and tube formation. Immunoblotting was used to analyse phosphorylation of signalling kinases. Effects in vivo were assayed using Matrigel plug and xenograft mouse models.

Key results

PPemd26 significantly inhibited VEGF-A-induced proliferation, mi

SUBMITTER: Huang SW 

PROVIDER: S-EPMC4290713 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature