Assessment of an efficient xeno-free culture system of human periodontal ligament stem cells.

ABSTRACT: The possibility of transplanting adult stem cells into damaged organs has opened new prospects for the treatment of several human pathologies. The purpose of this study was to develop a culture system for the expansion and production of human Periodontal Ligament Stem Cells (hPDLSCs) using a new xeno-free media formulation and ensuring the maintenance of the stem cells features comprising the multiple passage expansion, mesengenic lineage differentiation, cellular phenotype, and genomic stability, essential elements for conforming to translation to cell therapy. Somatic stem cells were isolated from the human periodontium using a minimally invasive periodontal access flap surgery in healthy donors. Expanded hPDLSCs in a xeno-free culture showed the morphological features of stem cells, exp

SUBMITTER: Trubiani O 

PROVIDER: S-EPMC4290797 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature