Combination of an unbiased amplification method and a resequencing microarray for detecting and genotyping equine arteritis virus.

ABSTRACT: This study shows that an unbiased amplification method applied to equine arteritis virus RNA significantly improves the sensitivity of the real-time reverse transcription-quantitative PCR (RT-qPCR) recommended by the World Organization for Animal Health. Twelve viral RNAs amplified using this method were hybridized on a high-density resequencing microarray for effective viral characterization.

SUBMITTER: Hans A 

PROVIDER: S-EPMC4290937 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature