Mining soil metagenomes to better understand the evolution of natural product structural diversity: pentangular polyphenols as a case study.

ABSTRACT: Sequence-guided mining of metagenomic libraries provides a means of recovering specific natural product gene clusters of interest from the environment. In this study, we use ketosynthase gene (KS) PCR amplicon sequences (sequence tags) to explore the structural and biosynthetic diversities of pentangular polyphenols (PP). In phylogenetic analyses, eDNA-derived sequence tags often fall between closely related clades that are associated with gene clusters known to encode distinct chemotypes. We show that these common "intermediate" sequence tags are useful for guiding the discovery of not only novel bioactive metabolites but also collections of closely related gene clusters that can provide new insights into the evolution of natural product structural diversity. Gene clusters corresponding t

SUBMITTER: Kang HS 

PROVIDER: S-EPMC4291760 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature