De novo amino acid biosynthesis contributes to salmonella enterica growth in Alfalfa seedling exudates.

ABSTRACT: Salmonella enterica is a member of the plant microbiome. Growth of S. enterica in sprouting-seed exudates is rapid; however, the active metabolic networks essential in this environment are unknown. To examine the metabolic requirements of S. enterica during growth in sprouting-seed exudates, we inoculated alfalfa seeds and identified 305 S. enterica proteins extracted 24 h postinoculation from planktonic cells. Over half the proteins had known metabolic functions, and they are involved in over one-quarter of the known metabolic reactions. Ion and metabolite transport accounted for the majority of detected reactions. Proteins involved in amino acid transport and metabolism were highly represented, suggesting that amino acid metabolic networks may be important for S. enterica growth in assoc

SUBMITTER: Kwan G 

PROVIDER: S-EPMC4292483 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature