MRNA destabilization is the dominant effect of mammalian microRNAs by the time substantial repression ensues.

ABSTRACT: MicroRNAs (miRNAs) regulate target mRNAs through a combination of translational repression and mRNA destabilization, with mRNA destabilization dominating at steady state in the few contexts examined globally. Here, we extend the global steady-state measurements to additional mammalian contexts and find that regardless of the miRNA, cell type, growth condition, or translational state, mRNA destabilization explains most (66%->90%) miRNA-mediated repression. We also determine the relative dynamics of translational repression and mRNA destabilization for endogenous mRNAs as a miRNA is induced. Although translational repression occurs rapidly, its effect is relatively weak, such that by the time consequential repression ensues, the effect of mRNA destabilization dominates. These results imply t

SUBMITTER: Eichhorn SW 

PROVIDER: S-EPMC4292926 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature