Morphine-induced internalization of the L83I mutant of the rat μ-opioid receptor.

ABSTRACT:

Background and purpose

Naturally occurring single-nucleotide polymorphisms (SNPs) within GPCRs can result in alterations in various pharmacological parameters. Understanding the regulation and function of endocytic trafficking of the μ-opioid receptor (MOP receptor) is of great importance given its implication in the development of opioid tolerance. This study has compared the agonist-dependent trafficking and signalling of L83I, the rat orthologue of a naturally occurring variant of the MOP receptor.

Experimental approach

Cell surface elisa, confocal microscopy and immunoprecipitation assays were used to characterize the trafficking properties of the MOP-L83I variant in comparison with the wild-type receptor in HEK 293 cells. Functional assays were used to compare the abili

SUBMITTER: Cooke AE 

PROVIDER: S-EPMC4292971 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature