Genomic Investigations unmask Mycoplasma amphoriforme, a new respiratory pathogen.

ABSTRACT:

Background

Mycoplasma amphoriforme has been associated with infection in patients with primary antibody deficiency (PAD). Little is known about the natural history of infection with this organism and its ability to be transmitted in the community.

Methods

The bacterial load was estimated in sequential sputum samples from 9 patients by quantitative polymerase chain reaction. The genomes of all available isolates, originating from patients in the United Kingdom, France, and Tunisia, were sequenced along with the type strain. Genomic data were assembled and annotated, and a high-resolution phylogenetic tree was constructed.

Results

By using high-resolution whole-genome sequencing (WGS) data, we show that patients can be chronically infected with M. amphoriforme manifesti

SUBMITTER: Gillespie SH 

PROVIDER: S-EPMC4293396 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature