A chloroplast genomic strategy for designing taxon specific DNA mini-barcodes: a case study on ginsengs.

ABSTRACT:

Background

Universal conventional DNA barcodes will become more and more popular in biological material identifications. However, in many cases such as processed medicines or canned food, the universal conventional barcodes are unnecessary and/or inapplicable due to DNA degradation. DNA mini-barcode is a solution for such specific purposes. Here we exemplify how to develop the best mini-barcodes for specific taxa using the ginseng genus (Panax) as an example.

Results

The chloroplast genome of P. notoginseng was sequenced. The genome was compared with that of P. ginseng. Regions of the highest variability were sought out. The shortest lengths which had the same discrimination powers of conventional lengths were considered the best mini-barcodes. The results showed that the ch

SUBMITTER: Dong W 

PROVIDER: S-EPMC4293818 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature