Optimized cell transplantation using adult rag2 mutant zebrafish.

ABSTRACT: Cell transplantation into adult zebrafish has lagged behind mouse models owing to the lack of immunocompromised strains. Here we have created rag2(E450fs) mutant zebrafish that have reduced numbers of functional T and B cells but are viable and fecund. Mutant fish engraft muscle, blood stem cells and various cancers. rag2(E450fs) mutant zebrafish are the first immunocompromised zebrafish model that permits robust, long-term engraftment of multiple tissues and cancer.

SUBMITTER: Tang Q 

PROVIDER: S-EPMC4294527 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature