Activation of intervertebral disc cells by co-culture with notochordal cells, conditioned medium and hypoxia.

ABSTRACT:

Background

Notochordal cells (NC) remain in the focus of research for regenerative therapy for the degenerated intervertebral disc (IVD) due to their progenitor status. Recent findings suggested their regenerative action on more mature disc cells, presumably by the secretion of specific factors, which has been described as notochordal cell conditioned medium (NCCM). The aim of this study was to determine NC culture conditions (2D/3D, fetal calf serum, oxygen level) that lead to significant IVD cell activation in an indirect co-culture system under normoxia and hypoxia (2% oxygen).

Methods

Porcine NC was kept in 2D monolayer and in 3D alginate bead culture to identify a suitable culture system for these cells. To test stimulating effects of NC, co-cultures of NC and bovine de

SUBMITTER: Gantenbein B 

PROVIDER: S-EPMC4295479 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature